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Total Antioxidant Capacity Bovine ELISA Kits / T - AOC Antibody ELISA Kit

Categories Bovine ELISA Kits
Brand Name: BT Lab
Model Number: Cat.No E0384Bo
Certification: CE, ISO9001:2005, MSDS
Place of Origin: Shanghai, China
MOQ: 1 kit
Price: Negotiation
Payment Terms: Western Union, T/T
Supply Ability: In Stock
Delivery Time: 1-3 business days, bulk order within one week
Packaging Details: Wrapped with ice pack and styrofoam package
Target Protein: Total Antioxidant Capacity
Shipping: DHL/FedEX
Assay Time: 2 hours
Discount: Available
Standard Curve Range: 0.1U/ml - 40U/ml
Assay Principle: Sandwich
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    Total Antioxidant Capacity Bovine ELISA Kits / T - AOC Antibody ELISA Kit

    Bovine Total Antioxidant Capacity ELISA Kit with High Spesificity and Sensitivity

    Cat.No E0384Bo


    Assay Principle

    This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Bovine T-AOC antibody. T-AOC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine T-AOC Antibody is added and binds to T-AOC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated T-AOC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine T-AOC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.


    Reagent Provided

    ComponentsQuantity

    Standard Solution (48U/ml)

    0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (30x)20ml x1
    Biotinylated Bovine T-AOC Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Specimen Collection

    Serum Allow serum to clot for 10-20 minutes at room temperature. Centrifuge at 2000-3000 RPM for 20 minutes.


    Plasma Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 2000-3000 RPM at 2 - 8°C within 30 minutes of collection.


    Urine Collect by sterile tube. Centrifuge at 2000-3000 RPM for approximately 20 minutes. When collecting pleuroperitoneal fluid and cerebrospinal fluid, please follow the procedures above-mentioned.


    Cell Culture Supernatant Collect by sterile tubes when examining secrete components. Centrifuge at 2000-3000 RPM for approximately 20 minutes. Collect the supernatants carefully. When examining the components within the cell, use PBS (pH 7.2-7.4) to dilute cell suspension to the cell concentration of approximately 1 million/ml. Damage cells through repeated freeze-thaw cycles to let out the inside components. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    Tissue Rinse tissues in PBS (pH 7.4) to remove excess blood thoroughly and weigh before homogenization. Mince tissues and homogenize them in PBS (pH7.4) with a glass homogenizer on ice. Thaw at 2-8°C or freeze at -20°C. Centrifuge at 2000-3000 RPM for approximately 20 minutes.


    Reagent Preparation

    All reagents should be brought to room temperature before use.

    Standard Reconstitute the 120μl of the standard (48U/ml) with 120μl of standard diluent to generate a 24U/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (24U/ml) 1:2 with standard diluent to produce 12U/ml, 6U/ml, 3U/ml and 1.5U/ml solutions. Standard diluent serves as the zero standard(0 U/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:


    24U/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
    12U/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    6U/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    3U/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    1.5U/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    48U/ml24U/ml12U/ml6U/ml3U/ml1.5U/ml

    Product Tags:

    elisa test kit

      

    elisa assay kit

      
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