Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme 170u /
Carboxypeptidase B (EC 126.96.36.199) catalyzes hydrolysis of the basic
amino acids lysine, arginine and ornithine from the C-terminal end
of polypeptides. The Mw was found to be 33.8 kda, optimun pH-7.9,
and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and
ornithine. The enzyme is not inhibited
by di-isopropylfluorophosphate (DFP), but it is inhibited by metal
chelating agents, e.g., EDTA,1,10-phenanthroline.
Synonyms: Carboxypeptidase B, Cpb1, Cpb.
Animal origin free:YaxinBio recombinant carboxypeptidase B belongs
to the AOF level 3, eliminate the risk of virus presence, or any
other potential adventitious agents found in animal-derived
Stability:A sterile recombinant carboxypeptidase B lyophilized
eliminates the risk of contamination and decreases the chances of
activity loss in the process of transport and storage.
1) Recombinant carboxypeptidase B provides increased specific
activity and eliminates contaminating proteases activities found in
extracted enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and
3)Less than 10ppm of recombinant trypsin.
35% ～ 60%
Single main band on SDS-PAGE
Less than 10 ppm of recombinant trypsin.
One Unit of carboxypeptidase B activity hydrolyzes one micromole of
hippuryl-L-arginine per minute at 25℃, pH 7.65.
To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM
Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000
(w/w), the optimum pH is pH 7-9.
Recommend recombinant carboxypeptidase B lyophilized should be
stored under 2℃-8℃ in sealed container. It is stable within 24
months.After dissolved, it should be stored under -20℃, It is
stable within 24 months and above 90% activity remained after 10
times repeated freezing and thawing.