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Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme 170u / mg Pro

Categories Recombinant Carboxypeptidase B
Brand Name: YaxinBio
Model Number: RCPB01
Certification: NSF ISO 9001
Place of Origin: China
MOQ: 10mg
Payment Terms: T/T
Packaging Details: ice packaging
Price: 150$/10mg
Supply Ability: 1000 per week
Purified by: HPLC
Physical form: White or white-like lyophilized powder
Additives: Tris salts, NaCl salts and carbohydrates
Cat. No.: RCPB01
Specific activity: ≥170 u/mg pro
Purity: > 95%
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    Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme 170u / mg Pro

    Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme 170u / mg Pro


    Description


    Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 33.8 kda, optimun pH-7.9,

    and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited

    by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA,1,10-phenanthroline.

    Synonyms: Carboxypeptidase B, Cpb1, Cpb.


    Advantages


    Animal origin free:YaxinBio recombinant carboxypeptidase B belongs to the AOF level 3, eliminate the risk of virus presence, or any other potential adventitious agents found in animal-derived carboxypeptitase B

    Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.

    High purity:

    1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.

    2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.

    3)Less than 10ppm of recombinant trypsin.


    Main Features


    Source

    E. Coli

    Protein content

    35% ~ 60%

    Purity

    Single main band on SDS-PAGE

    M.W.

    33.8kD

    Contaminant activity

    Less than 10 ppm of recombinant trypsin.


    Unit Definition


    One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.


    Recommend Usage


    To prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


    Storage Instructions


    Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Product Tags:

    recombinant insulin

      

    carboxypeptidase b

      
    Quality Carboxypeptidase B for Mass Spectrometry Recombinant Enzyme 170u / mg Pro for sale
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