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General Acetylcholine (ACH) ELISA Kit

Country/Region china
Company Wuxi Donglin Sci & Tech Development Co.,Ltd
Categories ELISA Reagents
Telephone +86-510-82732223,778-477-6628
ICP License Issued by the Chinese Ministry
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    General Acetylcholine (ACH) ELISA Kit

    General Acetylcholine (ACH) ELISA Kit

    0

    two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:General Acetylcholine (ACH) ELISA Kit
    Method:Competition
    Synonyms:
    Detection range:15.6-1,000pg/mL
    Reactivity:ACH
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-ACH-Ge (traditional)DLR-ACH-Ge (ready-to-use)
    Assay length1-2.5Hours1-2.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    1. Overview
    Function: Acetylcholine has functions both in the peripheral nervous system (PNS) and in the central nervous system (CNS) as a neuromodulator. Its receptors have very high binding constants. In the peripheral nervous system, acetylcholine activates muscles, and is a major neurotransmitter in the autonomic nervous system. In the central nervous system, acetylcholine and the associated neurons form a neurotransmitter system, the cholinergic system, which tends to cause anti-excitatory actions.
    2. Features
    INTENDED USE
    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ACH in serum, plasma, tissue homogenates and other biological fluids.
    DETECTION RANGE
    15.6-1000pg/mL. The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL.
    SENSITIVITY
    The minimum detectable dose of ACH is typically less than 6.8pg/mL.
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
    SPECIFICITY
    This assay has high sensitivity and excellent specificity for detection of ACH.
    No significant cross-reactivity or interference between ACH and analogues was observed.
    Note: Limited by current skills and knowledge, it is impossible to perform all possible cross-reactivity detection tests between ACH and all analogues, therefore, cross reactivity may still exist.
    IMPORTANT NOTES
    1. Limited by the current conditions and scientific technology, it is impossible to conduct comprehensive identification and analysis tests on the raw materials provided by suppliers. As a result, it is possible there are some qualitative and/or technical risks.
    2. The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    3. Kits from different batches may be a little different in detection range, sensitivity and color developing time. Please perform the experiment exactly according to the instruction manual included in your kit. Electronic ones on our website are for reference only.
    4. Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    5. Protect all reagents from strong light during storage and incubation. All bottle caps of reagents should be closed tightly to prevent evaporation of liquids and contamination by microorganisms.
    6. There may be a foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    7. Incorrect procedures during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D
    Product Tags:

    detection reagent

      

    bottle caps

      

    raw materials

      
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